The dose of the 0 . 10 , 0 . 15mmol / l cla had no difference . by mtt , determinied od after oil red o staining to investigate the effect of the cla , la , cla + la on the proliferation and differentiation of rat preadipocyte and adipocyte 通過(guò)mtt比色、油紅o染色od值測(cè)定等研究手段探討了cla 、 la 、 cla + la對(duì)大鼠前體脂肪細(xì)胞增殖與分化的影響。
Primary culture of rat preadipocyte were prepared from the epididymal , inguinal and perirenal the fat pads of male normal , healthy , 15 - 20 days sprague - dawley rats . the preadipocyte grew better under the condition of 37 , 95 % humidity , 5 % co2 , ph 7 . 0 - 7 . 2 , centrifuged at 1000r / min , m199medium , and 10 % fetal bo vine serum , seeded at a density of 4 l04 , 5 l04 , / cm2 . oil red o staining was the special method to distinguish adipocyte from other cells , gimsa and he could determine the stage of the adiopcyte differentiation through the number of lipid drop , size and the position of the nucleolus of the staining fat cell 經(jīng)過(guò)多次實(shí)驗(yàn),確定本實(shí)驗(yàn)室大鼠前體脂肪細(xì)胞的最佳培養(yǎng)條件是:溫度為37 ,濕度為95 , co _ 2濃度為5 , ph值為7 . 0 7 . 2 ,離心力為1000r / min ,培養(yǎng)基為m _ ( 199 )培養(yǎng)基,胎牛血清濃度為10 ,合適細(xì)胞接種密度為4 10 ~ 4 、 5 10 ~ 4個(gè)/ cm ~ 2 ,染色結(jié)果表明:油紅o染色是鑒定脂肪細(xì)胞的特異方法, gimsa和he染色可根據(jù)不同區(qū)域染色程度、著色差別判斷細(xì)胞核的位置及脂滴大小、多少,觀察大鼠前體脂肪細(xì)胞分化過(guò)程中的形態(tài)變化,進(jìn)而確定脂肪細(xì)胞的分化階段。